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 ORIGINAL ARTICLE
Year : 2016  |  Volume : 53  |  Issue : 3  |  Page : 345-348

Epidermal growth factor receptor expression in gastric tumors and its relationship with the germline polymorphisms − 216 G>T, −191 C>A, (CA) n IVS1, and R521K


1 División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social; Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Nextipac, Jalisco, ; Departamento de Ciencias Biológicas, División de Ciencias Biológicas y de la Salud, Centro Universitario de la Costa, Universidad de Guadalajara, Delegación Ixtapa, Puerto Vallarta, México
2 División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social; Doctorado en Genética Humana, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Nextipac, Jalisco, México
3 División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, México
4 Departamento de Biología Celular y Molecular, Laboratorio de Inmunobiología, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Nextipac, Jalisco, México
5 Departamento de Enseñanza y Capacitación, Instituto Jalisciense de Cancerología, Guadalajara, México
6 Departamento de Gastroenterología, Servicio de Endoscopías, Hospital de Especialidades del Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, México

Correspondence Address:
JY Sanchez-Lopez
División de Genética, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social
México
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0019-509X.200648

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BACKGROUND: Gastric cancer (GC) is the third worldwide leading cause of cancer-related death affecting both sexes. The aberrant expression of epidermal growth factor receptor (EGFR) gene has been detected in many human epithelial malignancies and linked to advanced disease, more aggressive phenotype, and poor prognosis. AIMS: To analyze the relation that the expression of EGFR in gastric tumors holds with pathological characteristics and with the germline polymorphisms −216 G>T, −191 C>A, (CA) n IVS1, and R521K. MATERIALS AND METHODS: We studied 22 biopsies from gastric tumors obtained by endoscopy. EGFR expression was determined by relative quantification real-time polymerase chain reaction with the glyceraldehyde-3-phosphate dehydrogenase reference gene (as for messenger RNA [mRNA]) and by immunohistochemistry (IHC) (as for protein). EGFR germline polymorphisms were analyzed by sequencing, GeneScan, and restriction fragment length polymorphisms. RESULTS: EGFR mRNA expression was increased (>2-fold) in 13.6% of GC cases, decreased (<0.5-fold) in 68.2%, and normal in 18.2%; overexpression was related to well-differentiated gastric tumors, whereas underexpression was linked to moderate or poorly differentiated gastric tumors (P < 0.001). EGFR protein expression was high (IHC 2+ and 3+) in 29.4% of gastric tumors and was normal or low (score 0 to 1+) in 70.6% cases. EGFR expression, in both mRNA and protein, was not related to any EGFR polymorphism (P > 0.05). CONCLUSIONS: Most gastric tumors showed low EGFR expression (mRNA and protein), whereas EGFR overexpression was related to well-differentiated gastric tumors. Furthermore, germinal polymorphisms −216, −191, (CA) n IVS1, and R521K were not related to EGFR expression (mRNA or protein).






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