| ORIGINAL ARTICLE |
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| Year : 2015 | Volume
: 52
| Issue : 6 | Page : 47-55 |
Transcatheter arterial infusion chemotherapy increases expression level of miR-142-5p in stage III colorectal cancer
D Shi1, B Zhai1, Y Zheng2, R Ren2, M Han2, X Wang3
1 Department of Interventional Oncology, Renji Hospital Shanghai Jiaotong University School of Medicine,160 Pujian Rd., Pudong, Shanghai 200127, China
2 State Key Laboratory of Genetic Engineering and Institute of Developmental Biology and Molecular Medicine School of Life Sciences, Fudan University, 220 Handan Rd., Shanghai 200433, China
3 Shanghai Institute of Medical Image Research, 180 Fenglin Rd., Shanghai 200032, China
Correspondence Address:
B Zhai
Department of Interventional Oncology, Renji Hospital Shanghai Jiaotong University School of Medicine,160 Pujian Rd., Pudong, Shanghai 200127
China
X Wang
Shanghai Institute of Medical Image Research, 180 Fenglin Rd., Shanghai 200032
China
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0019-509X.172513
Objective: To investigate the expression level of miR-142-5p and its potential target gene endothelial PAS domain protein 1(EPAS1) in Stage III colorectal cancer during Transcatheter arterial infusion chemotherapy (TAI). Materials and Methods: Illumina high-throughput sequencing was used to obtain miRNA expression profiles of paired tumor and adjacent normal tissues from one patient received TAI 1 week before the operation and another patient directly underwent an operation. The expression levels of miR-142-5p was measured with both high-throughput sequencing and quantitative real time-polymerase chain reaction. Results: The expression levels of miR-142-5p, were significantly reduced in tumor tissues of stage III CRC, then significantly increased in tumor tissues receiving TAI and higher than tumor tissues without TAI. The apoptosis rate of HT-29 colon cancer cells was mildly increased after transfection with pre-miR-142. miR-142-5p could bind directly to the 3′untranslated region of endothelial PAS domain protein 1 and reduce its expression. Conclusions: miR-142-5p is a potential tumor suppressor in CRC and is upregulated in tumor tissues after TAI, suggesting its potential clinical values for testing the functionality of TAI and predicting the progress of CRC.
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