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Figure 2: Effects of SPRY4-IT1 expression on esophageal squamous cell carcinoma cell viability. (a) After transfection with si-SPRY4-IT1, quantitative reverse transcriptase polymerase chain reaction analysis was performed to detect the knockdown effect. (b) Cell counting kit-8 assay was performed after knockdown of SPRY4-IT1 to determine TE-13 cell proliferation. (c) Clonogenic survival assay was performed after knockdown of SPRY4-IT1 to determine TE-13 cell survival. The results were expressed as mean ± standard deviation of three individual experiments, **P < 0.01

Figure 2: Effects of SPRY4-IT1 expression on esophageal squamous cell carcinoma cell viability. (a) After transfection with si-SPRY4-IT1, quantitative reverse transcriptase polymerase chain reaction analysis was performed to detect the knockdown effect. (b) Cell counting kit-8 assay was performed after knockdown of SPRY4-IT1 to determine TE-13 cell proliferation. (c) Clonogenic survival assay was performed after knockdown of SPRY4-IT1 to determine TE-13 cell survival. The results were expressed as mean ± standard deviation of three individual experiments, **<i>P</i> < 0.01